Random amplified polymorphic DNA (RAPD) analysis is a technique based on the polymerase chain reaction (PCR) amplification of discrete genome regions with short oligonucleotide primers of arbitrary sequence (WILLIAMS et al., 1990). The method is simple an
Limitations of RAPD. Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish whether a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). Co-dominant RAPD markers, observed as different-sized
These fast and specific methodologies include: DNA hybridization techniques, karyotype analysis, Random Amplified Polymorphic DNA (RAPD), chromosomal DNA Restriction Fragment Length Polymorphism ...
Post a Comment:
Showing 0 Comments: