MES (0.5 M, pH 6) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. MES buffer is a Good's buffer that is remarkably stable both chemically and enzymatically. It has minimal UV absorbance and is often u

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Make a 0.1M solution of the acid form of MES. Measure the pH. If the pH is much lower than pH4.5 then you can add the solution to your buffer which is at pH6.1.