How to Calculate Km and Vmax using Lineweaver Burk Plot

In this video I have explained how to calculate Km and Vmax of an enzyme in Lineweaver Burk double reciprocal plot.

The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km. It also gives a quick, visual impression of the different forms of enzyme inhibition.

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