How to Harvest ACL Hamstring Graft (Step by Step Surgical Technique)

Surgical Technique showing surgical procedure showing how to harvest hamstring autograft.

The hamstrings make up the bulk of the muscles in back of the thigh. The hamstrings are formed by three muscles and their tendons: the semitendinosus, semimembranosus, and biceps femoris. The top of the hamstrings connects to the ischial tuberosity, the small bony projection on the bottom of the pelvis, just below the buttocks. (There is one ischial tuberosity on the left and one on the right.)

The hamstring muscles run down the back of the thigh. Their tendons cross the knee joint and connect on each side of the tibia. The graft used in ACL reconstruction is taken from the hamstring tendon (semitendinosus) along the inside part of the thigh and knee. Dr. Vinay Singh also commonly includes a tendon just next to the semitendinousus, called the gracilis.

The hamstrings function by pulling the leg backward and by propelling the body forward while walking or running. This movement is called hip extension. The hamstrings also bend the knees, a motion called knee flexion.