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How to use Primer-Blast to create PCR primers

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How to use Primer-Blast to create PCR primers

86,830 views July 28, 2020 5:12

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ChannelDrDavidSmith

Description from the creator

This video gives a worked example of how to use the NCBI Primer-BLAST tool to work out which primers you will need for PCR.

The link to the tool is here: ncbi.nlm.nih.gov/tools/primer-blast/index.cgi

The DNA sequence used is here:

ATCTCAAGATCGTGCTGCAGACCAAGTGATCATACGAGATCTCTCGAATTCATGCATCTTCCGAGTCCCACTGACAGCAACTTCTATCGGGTGAATTGCGACGAAGTAGGTGGCGAAGCCTTGGGTCGCGCAGTCTTTCCGTCGATTGTCGGGCGTCCACGTCGTCTGACCAGTCTGGTGCGCGAAACGTTCATCACCGGCTTAGACGCTCCGCGTGGTGTGGTCGATTCGGAGGATCTGGAGCTGAACATCTCTCGGTTAGGGATTCACGAGGACAGCCAGAATCGCTTTGCGGATCTCTCAGAAGCAGCCAATCGCGCACAAGCGGAACTGGAAGCTCAGGAACTGCAGCGCGATAGCCAGGATGCCGGAGGTTTTGGCCCTGAGGATCGTTGCGAAGTGGGCTATACAGGCGTACGCGTTACGCATGCGGTTGTGACCGTTCCGGCGTACTTCAACGATGCGCAACGCGCCTGGCTCGAGCACCACCACCACCACCACTGAGATCCATCGTGAGATGCAAA GCCCATTTACCGTACCACCATCGTGATGTCTGGA

About this tutorial

DrDavidSmith published this 5:12 video tutorial on July 28, 2020. It has been watched 86,830 times on YouTube.

Category
Education
Duration
5:12
Published
July 28, 2020
Views
86,830
Likes
785

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