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How To Create Real-Time PCR Primers Using Primer-BLAST

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How To Create Real-Time PCR Primers Using Primer-BLAST

156,086 views October 28, 2018 12:26

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ChannelTop Tip Bio

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This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.

🎓 MASTERING QPCR ONLINE COURSE 🎓
courses.toptipbio.com/p/mastering-qpcr

In this video tutorial, I will show you how to create real-time PCR primers by using NCBI's Primer-BLAST free online software.

The Primer-BLAST software is great since it uses the Primer3 algorithm to design optimal primers, while simultaneously blasting these sequences on the genome. This ensures the primers will not amplify off-target regions.

THE ONLINE GUIDE
toptipbio.com/real-time-pcr-primer-blast/

NCBI WEBSITE
ncbi.nlm.nih.gov/

VIDEO BREAKDOWN
Step 1: Finding your gene variant (00:18)
Step 2: Loading Primer-BLAST (01:53)
Step 3: The Primer-BLAST settings (02:22)
Step 4: The primer reports (08:40)

MORE HELPFUL HINTS & TIPS
toptipbio.com/

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About this tutorial

Top Tip Bio published this 12:26 video tutorial on October 28, 2018. It has been watched 156,086 times on YouTube.

Duration
12:26
Published
October 28, 2018
Views
156,086
Likes
2,736

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