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How to culture Methanotrophs

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How to culture Methanotrophs

2,481 views October 31, 2018 9:44

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ChannelC1 Biocatalysis

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Culturing of the model methanotroph _Methylmicrobium alcaliphilum_ 20Z. Visit our lab website for more information on C1 Biocatalysis sci.sdsu.edu/kalyuzhlab/

P3% Media:
KNO3 1 g/L
MgSO4 x 7H2O 0.2 g/L
CaCl2 x 2H2O 0.02 g/L
NaCl 30 g/L
Trace solution (1000x) 1 mL/L
Water (distilled deionized water) QS 1L

Agar (solid) 15 g/L

Phosphate Solution (pH 6.8)
KH2PO4 5.44 g/L
Na2HPO4 5.68 g/L

Carbonate Solution (pH 8.8-9.0)
NaHCO3 75.6 g/L
Na2CO3 10.5 g/L

P media, Trace mineral solution and Phosphate solution should be sterilized by autoclaving @ 121℃ for 30 minutes. Carbonate solution should be sterilized by 0.2µm filtration.

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C1 Biocatalysis published this 9:44 video tutorial on October 31, 2018. It has been watched 2,481 times on YouTube.

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9:44
Published
October 31, 2018
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2,481
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