Primer Premier follows all the guidelines specified for PCR primer design. Primer Premier can be used to design primers for single templates, alignments, degenerate primer design, restriction enzyme analysis. contig analysis and design of sequencing prime

Go to the Primer BLAST submission form. Enter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template section of the form. If the NCBI mRNA reference sequence accession number is used, the tool will au

Get accession for sequences found in a database. Primer-BLAST identifies the template better by RefSeq accession than by raw DNA sequence. Another advantage of RefSeq accession is functions related to exon/intron are available only when inputting RefSeq m