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Converting your 454 raw data (*sff ) to *fastq files + clipping (Mitogenome Tutorial 2.2a)

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Converting your 454 raw data (*sff ) to *fastq files + clipping (Mitogenome Tutorial 2.2a)

3,840 views October 28, 2014 7:08

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Channelluckylion

Description from the creator

In this tutorial we will learn how to convert the raw data from your 454 sequencer (sff file) using the script sff_ectract. We will also apply some quality clipping, so our reads don't have to many sequencing errors. We save the NGS data as fastq files, which includes sequence quality information and can be open in Geneious and FastQC for a quick quality check. Also make sure to write your labnotebook while following this course, documentation is really impotent!

Download the script we need for data extraction here:
bioinf.comav.upv.es/sff_extract/download.html

You find the out the complete course documentation and additional written documentation on the tutorial here:

About this tutorial

luckylion published this 7:08 video tutorial on October 28, 2014. It has been watched 3,840 times on YouTube.

Channel
luckylion
Duration
7:08
Published
October 28, 2014
Views
3,840
Likes
18

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