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How to Filter Buffers (Vacuum Filtration)

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How to Filter Buffers (Vacuum Filtration)

67,652 views October 28, 2010 3:47

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Channellabtricks

Description from the creator

In this video, Dan shows you how to properly assemble and use the vacuum filtration apparatus.

Sometimes you need to filter your buffers or other solutions before you use them. If you are using any automated liquid chromatography systems (such as AKTA or FPLC systems), then you need to filter your buffers before loading them on the columns.

Remember:
TO START: Attach tubing THEN turn on tap.
TO END: Remove tubing THEN turn off tap.

*If you have detergent in your buffer, bubbles will form during filtration -- make sure to continuously monitor and break the vacuum before the bubbles rise up to the level of the tubing.

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About this tutorial

labtricks published this 3:47 video tutorial on October 28, 2010. It has been watched 67,652 times on YouTube.

Channel
labtricks
Duration
3:47
Published
October 28, 2010
Views
67,652
Likes
431

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