How to isolate total RNA using magnetic bead–based technology

Learn how to perform a **total RNA extraction protocol** using magnetic bead–based technology for fast, reliable RNA isolation. In this video, we demonstrate how the MagMAX mirVana Total RNA Isolation Kit enables quantitative recovery of RNA from tissue and plasma samples, supporting both manual and automated workflows.

🔗 Learn more: thermofisher.com/us/en/home/life-science/rna.ht…

What You’ll Learn:
- The principles of bead-based isolation compared to filter-based RNA extraction.
- Step-by-step manual RNA extraction from tissue stored in RNAlater.
- Preparing reagents, lysis buffer, and magnetic bead binding steps.
- How to use a KingFisher Flex instrument for automated RNA purification.
- Applications of purified RNA in qPCR and other downstream analyses.

Materials Used:
• MagMAX™ mirVana™ Total RNA Isolation Kit
• Tissue samples
• Plasma samples
• Magnetic bead-based reagents
• RNA extraction workflow materials
• Manual and automated processing equipment

Steps Covered:
1. Prepare tissue or plasma samples.
2. Add magnetic bead-based extraction reagents.
3. Bind RNA to magnetic particles.
4. Wash and process samples according to protocol.
5. Elute purified RNA for downstream analysis.

📌 CHAPTERS:
00:00 – Introduction to Total RNA Extraction
00:40 – Manual RNA Extraction Workflow
02:00 – Reagent Preparation and Bead Binding
03:15 – Washing and Elution Steps
04:30 – Automated RNA Isolation with KingFisher Flex
06:00 – RNA Purification for qPCR and Storage

FAQs
Q: What is magnetic bead-based RNA extraction?
It is an RNA isolation approach that uses magnetic particles during sample processing.

Q: Can RNA be extracted from tissue and plasma samples?
RNA extraction workflows may be used with a variety of sample types, including tissue and plasma.

Q: Why is RNA extraction important?
RNA isolation is commonly performed before downstream molecular biology analyses.

Related Videos:
- Purify Circulating Cell-Free DNA → youtube.com/watch?v=xbjwRLoQwtw
- Qubit 4: RNA Quantitation → youtube.com/watch?v=pfVbbdu9-nc
- Principles of PCR → youtube.com/watch?v=VD5qEVTsjTc

Compliance
For Research Use Only. Not for diagnostic procedures.

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