How to plot a standard curve and determine the unknown concentration of a sample

This video describes how to plot data from a spectrophotometer and use that curve to determine a sample's unknown concentration.

👉 For more help with your graph plotting, see this video - youtube.com/watch?v=COoYXfgv40o&list=PLW8Zt…

In the video, you'll learn how to plot a standard curve and use it to determine the unknown concentration of a sample. The video will guide you step-by-step through the process using data from a spectrophotometer. However, the method can be applied to any equipment that provides readings for known concentrations. In the video, I plot the absorbance and concentration values, explain how to properly label your graph, and determine the best line to fit according to the Beer-Lambert law. Finally, you'll see how to use the graph to calculate the concentration of an unknown sample with a given absorbance. By the end of the video, you'll know how to plot data accurately and solve for unknown concentrations using a standard curve.

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Resources:
- 📗 - You can download a free graph drawing checklist at: maths.drnickmorris.com/graph.html
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- 📗 - Catchup Maths and Stats - amzn.to/3Bq6HqE (affiliate link)
- 📗 - Catchup Chemistry - amzn.to/3TMFoNm (affiliate link)

- 🌎 - You can practice your percentage weight by volume (w/v) calculations at: maths4biosciences.drnickmorris.com/?t=standardc…

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