How To Pour LB Agar Plates for pGLO Bacterial Transformation Kits

This video demonstrates how to pour the LB Agar plates for the #pGLO Bacterial Transformation Kit activities. Do NOT follow this protocol when preparing LB Agar plates for the pGLO Bacterial Transformation Kit for General Biology (17006991EDU).

For instructions to prepare LB/Agar plates for the pGLO Bacterial Transformation Kit for General Biology, please refer to the instructor guide found at bio-rad.com/en-us/product/pglo-bacterial-transf…


Using the pGLO Bacterial Transformation Kit, students can analyze the growth of bacteria on various media and examine the roles of external and internal factors in gene regulation.

Bio-Rad's exclusive pGLO plasmid is constructed with the jellyfish gene that encodes green fluorescent protein (GFP), an antibiotic-resistance gene that encodes β-lactamase protein, and the araC gene, encoding a regulator protein that turns the GFP gene on and off. Bacteria transformed with pGLO plasmid are selected by ampicillin resistance; when induced to express GFP, the microbes glow fluorescent green under UV light.

When students genetically reengineer bacteria with the genes from a bioluminescent jellyfish, they never forget the central mantra of molecular biology:

DNA ⇒ RNA ⇒ Protein ⇒ Trait — Green Fluorescence

For more information, visit bio-rad.com/pGLO

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