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How to prepare a GPC/SEC sample

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How to prepare a GPC/SEC sample

5,113 views May 25, 2020 6:01

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ChannelMalvern Panalytical

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In this video, we'll take you through a step-by-step guide on how to prepare GPC/SEC samples, perfect for beginners. Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) are powerful analytical techniques used to separate, identify, and quantify the components of a mixture based on their size. To get accurate results, it's crucial to prepare your samples correctly. We'll cover the essential steps to prepare your samples, from preparing the mobile phase to injecting your sample into the instrument, such as OMNISEC or TDA from Malvern Panalytical. The key considerations of GPC/SEC sample preparation are described in this demonstration video. Topics discussed include sample concentration, dissolution time and temperature, and sample solution filtration. By the end of this video, you'll be confident in preparing high-quality GPC/SEC samples, ensuring reliable and reproducible results in your research or quality control applications.

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Malvern Panalytical published this 6:01 video tutorial on May 25, 2020. It has been watched 5,113 times on YouTube.

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6:01
Published
May 25, 2020
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5,113
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Further reading

  • GPC Sample Preparation - Rice University ruf.rice.edu

    Check if polymer sample is prepared. If not, prepare polymer samples to be tested. · Target concentration is 50 mg-polymer/mL-solvent. · A minimum of 1 mL of polymer solution must be prepared. · Prepare samples in 5 mL…

  • Size exclusion chromatography (SEC) Gel Permeation ... waters.com

    GPC separates molecules in solution by their "effective size in solution." To prepare a sample for GPC analysis the resin is first dissolved in an appropriate solvent. Inside the gel permeation chromatograph, the dissol…

  • Sample Preparation and GPC/SEC/APC Method Development waters.com

    A. Sample Preparation for GPC/SEC/APC Analysis 1. Sample Types 2. Solvents 3. Filters B. GPC/SEC/APC Method Development 1. Eluent(s) / Mobile Phase(s) 2. Additives 3. Column(s) 4. Sample Loading 5. Flow Rate 6. Temperat…

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