How to Use video for Pierce Chromogenic Endotoxin Quant Kit

Endotoxin contamination is a major concern in proteomics sample preparation, especially when working with recombinant proteins, antibodies, or nucleic acids purified from Gram-negative bacteria. The **Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit** provides a fast, reliable way to detect and quantify endotoxins in your samples before downstream mass spectrometry analysis.

In this video, we walk through the step-by-step workflow:
- Preparing pyrogen-free consumables and equilibrating reagents.
- Setting up standards, blanks, and samples for the assay.
- Running the chromogenic reaction with Amebocyte Lysate (LAL).
- Measuring absorbance at 405 nm and generating a standard curve.
- Interpreting results across two sensitivity ranges (0.01–0.1 EU/mL and 0.1–1.0 EU/mL).

This kit is highly specific for endotoxins, with no interference from beta-glucans, and is designed to support consistent results for protein digestion, peptide clean-up, and other MS sample prep workflows.

🔗 Learn more: thermofisher.com/us/en/home/life-science/protei…

📌 Chapters:
00:00 – Introduction: Why Endotoxin Detection Matters
00:50 – Preparing Standards and Samples
02:10 – Adding Amebocyte Lysate (LAL) Reagent
03:20 – Running the Chromogenic Reaction
04:40 – Measuring Absorbance and Standard Curve Setup
06:00 – Sensitivity Ranges and Data Interpretation
07:15 – Applications in Proteomics and Mass Spec Sample Prep

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- Protein Purification Workflows (NuPAGE Novex) → youtube.com/watch?v=yy8f39XCQgs