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Learn EMSA (Electrophoretic Mobility Shift Assay) in 15 min - Tutorial & Protocol, Step by Step

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Learn EMSA (Electrophoretic Mobility Shift Assay) in 15 min - Tutorial & Protocol, Step by Step

20,600 views June 6, 2020 15:16

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ChannelSciGine

Description from the creator

Learn EMSA (Electrophoretic Mobility Shift Assay) in 15 min with our step by step tutorial where we go through this protocol from Nature:
ncbi.nlm.nih.gov/pmc/articles/PMC2757439/

Topics we cover include:
- What is EMSA?
- How can you use EMSA to determine DNA Protein interactions
- How do you prepare labeled DNA via fluorescence, biotin, or P32
- How do you setup and run a gel
- How do you do protein titrations to determine the ideal concentration for complex formation
- How do you analyze EMSA data

Related protocols you should check out:
- Gel Electrophoresis guide: youtube.com/watch?v=Dc7AQb1Glrg
- SDS Page Guide: youtube.com/watch?v=mabx-U5X9J8
- Promega protocol for DNA labeling with P32:

Scigine protocols for EMSA:
- scigine.com/method.php?ID=247886
- scigine.com/method.php?ID=2419
- scigine.com/method.php?ID=6527

About this tutorial

SciGine published this 15:16 video tutorial on June 6, 2020. It has been watched 20,600 times on YouTube.

Channel
SciGine
Duration
15:16
Published
June 6, 2020
Views
20,600
Likes
546

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