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ELISA Tutorial 1: How a Direct, Indirect and Sandwich ELISA Works

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ELISA Tutorial 1: How a Direct, Indirect and Sandwich ELISA Works

373,327 views October 25, 2013 9:07

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Channelprotocolplace

Description from the creator

Here, we discuss the concept behind ELISA (Enzyme-Linked Immunosorbent Assay). Three types of ELISA are discussed: the Direct ELISA, Indirect ELISA, and Sandwich ELISA.

Our ELISA tutorials are kindly sponsored by Calbiotech. Click here to see Calbiotech's ELISA kits: protocol-place.com/Calbiotech

This video and other protocols can be found at our website, the "Protocol Place" - protocol-place.com/

A full ELISA protocol can be found at protocol-place.com/assays/sandwich-elisa-protoc…

This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series:

ELISA Tutorial 1: Understand How an ELISA Works - youtu.be/nNjlBCnpGZ4

ELISA Tutorial 2: Coating and Blocking the ELISA Plate - youtu.be/AmG7FBolfdc

ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - youtu.be/darrx6F0wsg

ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - youtu.be/zI4khIJhCd8

ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - youtu.be/l9tO81ZCeRg

ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - youtu.be/5IqqpKSnXfI

Competitive ELISA Tutorial 1: How a Competitive ELISA Works - youtu.be/Kb26nQVMHds

Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - youtu.be/3E_U_4Z2dUc

Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - youtu.be/s2t0jiWxiDI

We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others!

Thanks for watching!

Youssef Farhat

Protocol Place: protocol-place.com

***Check out some of our other tutorials via the links below***

Competitive ELISA Tutorials - - - youtube.com/watch?v=Kb26nQVMHds&list=PLR4wf…

ELISA Tutorials - - - youtube.com/watch?v=nNjlBCnpGZ4&list=PLR4wf…

Gelatin Zymography Tutorials - - - youtube.com/watch?v=MF2sWQSaBWg&list=PLR4wf…

About this tutorial

protocolplace published this 9:07 video tutorial on October 25, 2013. It has been watched 373,327 times on YouTube.

Duration
9:07
Published
October 25, 2013
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373,327
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3,122

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Further reading

  • Types of ELISA | Bio-Rad bio-rad-antibodies.com

    This is known as an indirect sandwich ELISA. The key advantage of a sandwich ELISA is its high sensitivity; it is 2-5 times more sensitive than direct or indirect ELISAs. Sandwich ELISA also delivers high specificity as…

  • ELISA Basics Guide - Bio-Rad bio-rad-antibodies.com

    to as a direct sandwich ELISA . If the detection antibody used is unlabeled, a secondary enzyme-conjugated detection antibody is required . This is known as an indirect sandwich ELISA . The key advantage of a sandwich E…

  • ELISA Fundamental Principle, How It Works bosterbio.com

    Comparisons on Direct, Indirect, Sandwich, Competitive ELISA ELISAs can be performed with a number of modifications to the basic procedure: direct, indirect, sandwich or competitive. The key step, immobilization of the…

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