ELISA Tutorial 2: Coating and Blocking the ELISA Plate

Here, we illustrate the process and concept behind the initial steps of a Sandwich ELISA: coating an ELISA plate with a capture antibody and then blocking the plate with BSA or a detergent-based Blocking Buffer.

Our ELISA tutorials are kindly sponsored by Calbiotech. Click here to see Calbiotech's ELISA kits: protocol-place.com/Calbiotech

This video and other protocols can be found at our website, the "Protocol Place" - protocol-place.com/

A full ELISA protocol can be found at protocol-place.com/assays/sandwich-elisa-protoc…

This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series:

ELISA Tutorial 1: Understand How an ELISA Works - youtu.be/nNjlBCnpGZ4

ELISA Tutorial 2: Coating and Blocking the ELISA Plate - youtu.be/AmG7FBolfdc

ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - youtu.be/darrx6F0wsg

ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - youtu.be/zI4khIJhCd8

ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - youtu.be/l9tO81ZCeRg

ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - youtu.be/5IqqpKSnXfI

Competitive ELISA Tutorial 1: How a Competitive ELISA Works - youtu.be/Kb26nQVMHds

Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - youtu.be/3E_U_4Z2dUc

Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - youtu.be/s2t0jiWxiDI

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Thanks for watching!

Youssef Farhat

Protocol Place: protocol-place.com

***Check out some of our other tutorials via the links below***

Competitive ELISA Tutorials - - - youtube.com/watch?v=Kb26nQVMHds&list=PLR4wf…

ELISA Tutorials - - - youtube.com/watch?v=nNjlBCnpGZ4&list=PLR4wf…

Gelatin Zymography Tutorials - - - youtube.com/watch?v=MF2sWQSaBWg&list=PLR4wf…