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How to Transition hPSCs into mTeSR™ Plus: Plate Coating

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How to Transition hPSCs into mTeSR™ Plus: Plate Coating

15,632 views June 1, 2020 5:21

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ChannelSTEMCELLTechnologies

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Description from the creator

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This video will provide step by step instructions for plate coating.

0:00 - Intro
0:29 - Matrigel Plate Coating
2:42 - Vitronectin XF™ Plate Coating

Watch the other videos in the series below:

Video 2: Generation of Cell Aggregates/Passaging - youtu.be/oOdRca3Gw1M
Video 3: Maintenance and Morphological Assessment - youtu.be/3gtec8e1qSA

Learn more about mTeSR™ Plus hPSC maintenance media: bit.ly/3l2qJKt

View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus: bit.ly/3etrGJm

#PluripotentStemCells #CellCulture #mTeSRPlus

For a full list of products, as well as educational resources, visit our website: stemcell.com

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About this tutorial

STEMCELLTechnologies published this 5:21 video tutorial on June 1, 2020. It has been watched 15,632 times on YouTube.

Duration
5:21
Published
June 1, 2020
Views
15,632
Likes
87

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