How to do a proper cell culture quick check

Many fields of biomedical research, like cancer research, drug development and tissue engineering, require the use of living cells to perform a variety of assays. Mammalian cell cultures are an essential tool in biology because they allow rapid growth and proliferation of different cell types for experimental analysis. In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. When a cell line reaches about 80% confluence, the cells must be subcultured to ensure proper growth and health of the cells. 80% confluency means when 80% of the surface of a culture vessel is covered with cells. This video shows a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps.

More information about the Leica DMi1, the microscope used in the video: leica-microsystems.com/products/light-microscop…