How to perform a western blot dry transfer using the Invitrogen iBlot 2 Dry Blotting System

Master the **western blot transfer protocol** with the **Invitrogen iBlot 2 Dry Blotting System**, a bufferless, plug-and-play device designed for fast and reliable protein transfer. In this video, we walk through every step of the dry transfer process, from preparing gels and membranes to setting up the transfer stack and running the instrument.

You’ll learn:
- How the iBlot 2 system eliminates the need for traditional transfer buffers.
- Step-by-step instructions for transferring proteins to nitrocellulose or PVDF membranes.
- How to align transfer stacks, use filter papers, and ensure even transfer.
- Troubleshooting tips for common protein transfer issues.
- How to clean and maintain your iBlot 2 after use.

With preset protocols and customizable methods, the iBlot 2 provides speed and convenience while maintaining consistent, high-quality protein transfer for western blotting applications.

🔗 Learn more: thermofisher.com/us/en/home/life-science/protei…

thermofisher.com/us/en/home/life-science/protei…

📌 Chapters:
00:00 – Introduction to Western Blot Transfers
00:40 – Preparing Transfer Stacks and Gels
02:00 – Aligning Membranes and Removing Air Bubbles
03:20 – Running Preset or Custom Methods on iBlot 2
05:00 – Disassembling and Retrieving the Membrane
06:20 – Cleaning and Maintaining the iBlot 2

Related Videos:
- Invitrogen NuPAGE Novex Gel System → youtube.com/watch?v=yy8f39XCQgs
- EVOS M5000 Cell Imaging System → youtube.com/watch?v=_Am0q4gnfTE
- Capture Images with iBright → youtube.com/watch?v=54DunZtPc-0

#ThermoFisher #Invitrogen #WesternBlot