How to perform an isothermal amplification test with AmplifyRP?

Agdia-Biofords: How to Videos.

For exact instructions, please refer strictly to the protocol sent with the kit.

You will learn with this video how to do a rapid molecular test to detect plant pathogens.

AmplifyRP® testing platform uses a patented isothermal amplification technology, Recombinase Polymerase Amplification (RPA), which enables highly specific and sensitive DNA or RNA analysis of plant pathogens in any type of testing environnement.

AmplifyRP® technology achieves the sensitivity and specificity of PCR based testing methods, but has some clear advantages. Unlike PCR, AmplifyRP® testing formats do not require thermal cycling. The entire process is completed at a single operating temperature (39°C) using a portable hot block or fluorometer. Additionally, AmplifyRP® does not require a DNA or RNA purification step. Crude sample extracts can be prepared using a simple extraction buffer and can be tested directly. This makes the testing process very simple and saves the end user valuable time.

If you have any questions about using these kits, please contact Agdia-Biofords, Monday-Friday by phone (+33 (0)1 60 78 81 64) or by email ([email protected]).

www.agdia-biofords.com